Dynamics of Microbial Contamination of the Experimental Purulent Wound During Topical Treatment with Agents Containing Copper Nanoparticles

The effect of topical application of copper and zinc nanoparticle suspensions on the dynamics of bacterial contamination of the experimental purulent wound was studied. A model of purulent wound infected with polyantibiotic-resistant clinical strains of Staphylococcus aureus isolated from orthopedic trauma patients was obtained in 30 white male rats. The use of copper nanoparticle suspension ensured rapid elimination of the infectious agent contaminating the wound; zinc nanoparticles had a weaker antibacterial effect: the St. aureus culture was disseminated until day 12 (until day 20 in the control group). The quantitative study of microbial contamination of 1 g of wound tissue demonstrated that it decreased to 10 CFU/g in the experimental animals 2 treated with copper nanoparticles. The use of zinc nanoparticles to treat the wound resulted in a lesser decrease in the count of microbial bodies: the CFU/g value decreased to 10 CFU/ml on day 5, which also reliably differed 5 from the control group, where an increase in the degree of bacterial contamination of the wound was observed at this time. The studies revealed the high antibacterial activity of copper nanoparticle suspension against clinical polyantibiotic-resistant strains of St. aureus under conditions of experimental purulent would.